Mcherry-egfp-lc3b
WebmCherry-GFP-LC3B自噬双标系统的工作原理： 未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。. 当自噬体与溶酶体融合形成自噬 … Web12 aug. 2024 · mCherry (红光)-eGFP (绿光)-LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。 它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的 …
Mcherry-egfp-lc3b
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WebE (0)GFP-mCherry shows ideal properties for FLIM detection of FRET and yields high accuracy both in vitro and in vivo. Furthermore, the recently introduced phasor approach to FLIM is shown to yield straightforward and accurate two-photon FRET efficiency data even in suboptimal experimental conditions. http://www.biofeng.com/adjy/zhili/pBABE-puro%20mCherry-EGFP-LC3B.html
Webメーカー名：Cell Biolabs Inc. GFP-LC3 発現ベクターはオートファジーの研究にとても便利なツールです。 哺乳類細胞発現ベクター、レンチウイルス発現ベクターおよびレトロ … WebTHP1-Difluo™ hLC3 reporter cells are designed to monitor the autophagic flux by means of a fluorescent-labeled LC3B protein. These cells, derived from the THP-1 human …
WebmCherry-eGFP-LC3B, eGFP-p62, and Acridine Orange are co-localized with fluorescently-labeled chloroquine in AVs detected by OFACS. (A) Representative microscopy images of PC3 cells stably... WebQuantification of eGFP-mCherry-LC3B puncta G (n = 3). eGFP fluorescence was measured by flow cytometry in L6 cells stably expressing tandem fluorescent eGFP-mCherry …
Web本产品是一种可以表达mCherry-GFP-LC3B融合蛋白的重组腺病毒，可以用于感染细胞或组织后进行细胞自噬的检测。 感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿 …
Web8 jul. 2024 · Inmortalized Human Chondrocytes (T/C28a2) were employed to develop an autophagy reporter cell line using LC3 as a fluorescent reporter (mCherry-EGFP-LC3B) … lightbeam solutionsWebThe LC3B protein is a good marker to assess the autophagic flux since it is recruited from the cytosol to be associated to all the autophagic structures: the nascent autophagosome (called the isolation membrane), the complete autophagosome and the autolysosome. pbirdinthehandisworthtwointhWebArticle Snippet: GFP-tagged LC3B expressing UACC 903 cell line was generated using the pBABE-puro mCherry-EGFP-LC3B plasmid (Addgene plasmid #22418; Cambridge, … lightbeamer tbzWeb19 feb. 2024 · Flow cytometry software like Flow Jo can run a ratiometric calculation as a derived parameter, where autophagic flux is the mCherry signal divided by the GFP signal. This assay is simple and quick with a 1 … lightbeamer tower blitzWeb24 aug. 2024 · To further validate that ATL2 regulates only ER-phagy and not general autophagy, we used cells stably expressing mCherry-eGFP-LC3B to measure general autophagy flux by flow cytometry. We found that general autophagy is inhibited only in cells lacking ATG10 but not lacking ATL2 ( Fig. 2 H ). lightbeamers lyricsWeb说起自噬，不得不提的就是lc3蛋白啦，是微管相关蛋白轻链3（map-lc3）的简称，它全程参与了自噬形成，并且也被用作哺乳类自噬作用的标记蛋白，自噬发生初期，胞浆型lc3（ … lightbeam population healthWebmCherry-GFP-LC3B腺相关病毒. 四．维真自噬双标系统的工作原理. 未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。. 当自噬体与溶 … pbnabluffton